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dermal microvascular endothelial cells hdmec  (PromoCell)


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    PromoCell dermal microvascular endothelial cells hdmec
    Dermal Microvascular Endothelial Cells Hdmec, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hdmec+hdmec+cells/Human+Dermal+Microvascular+Endothelial+Cells+(HDMEC)+adult+donor/pm42237191-32-14-33
    Average 94 stars, based on 144 article reviews
    dermal microvascular endothelial cells hdmec - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Native C3 is activated without proteolytic cleavage by transformation to C3(H 2 O) on phospholipid-scrambled cell membranes
    Article Snippet: a. PMN: Blood was collected in 10 mL sodium heparin (17 IU/mL) tubes (BD Vacutainer) and diluted 1:2 in sterile PBS at RT followed by purification on Ficoll-Paque Plus density gradients (GE Healthcare, Uppsala, Sweden) to isolate PMN. .. The cells were diluted to 1 × 10 6 /mL in RPMI 1640 medium with L-glutamine (Invitrogen, Paisley, UK) and the cell purity and viability were checked with trypan blue using a Luna II automated cell counter (Logos, Biosystems, Gyeonggi-do, South Korea). b. HDMEC: HDMEC cells (PromoCell GmbH, Heidelberg, Germany) were culture in human endothelial cell medium (ECM [ 3 H Biomedical, Uppsala, Sweden]) containing 10% heat-inactivated FCS and the cell purity and viability were checked with trypan blue using a Luna II automated cell counter (Logos, Biosystems, Gyeonggi-do, South Korea). c. Apoptosis: For induction of apoptosis, PMN and HDMEC were incubated in 5 μM camptothecin (Sigma Aldrich) for 4h at 37°C. ..



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